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Col1a1tm1(tetO-RNAi:Rps19)Karl
Targeted Allele Detail
Nomenclature
Symbol: Col1a1tm1(tetO-RNAi:Rps19)Karl
Name: collagen, type I, alpha 1; targeted mutation 1, Stefan Karlsson
MGI ID: MGI:5304754
Synonyms: shRNA-B
Gene: Col1a1  Location: Chr11:94936224-94953042 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:179085
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Inducible, Inserted expressed sequence, Knockdown)
Mutation:    Insertion
 
Mutation detailsRMCE on KH2 cells inserts an shRNA targeting Rsp19 behind a tetracycline responsive element and CMV promoter replacing the Pou5f1 cassette in Col1a1tm2(tetO-Pou5f1)Jae into the 3'UTR. The level of knock down by this allele following doxycycline treatment is lower compared to mice expressing Col1a1tm2(tetO-RNAi:Rps19)Karl . (J:179085)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  68 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae.

References
Original:  J:179085 Jaako P, et al., Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia. Blood. 2011 Dec 1;118(23):6087-96
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory