Col1a1^{tm1(tetO-RNAi:Rps19)Karl}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-RNAi:Rps19)Karl}
• Name: collagen, type I, alpha 1; targeted mutation 1, Stefan Karlsson
• MGI ID: MGI:5304754
• Synonyms: shRNA-B
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-RNAi:Rps19)Karl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179085
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-RNAi:Rps19)Karl} involves 1 genes/genome features (Rps19) View all
• Mutation details: RMCE on KH2 cells inserts an shRNA targeting Rsp19 behind a tetracycline responsive element and CMV promoter replacing the Pou5f1 cassette in Col1a1^{tm2(tetO-Pou5f1)Jae} into the 3'UTR. The level of knock down by this allele following doxycycline treatment is lower compared to mice expressing Col1a1^{tm2(tetO-RNAi:Rps19)Karl} . (J:179085)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:179085 Jaako P, et al., Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia. Blood. 2011 Dec 1;118(23):6087-96
• All: 3 reference(s)