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Targeted Allele Detail
Symbol: Col1a1tm1(tetO-RNAi:Rps19)Karl
Name: collagen, type I, alpha 1; targeted mutation 1, Stefan Karlsson
MGI ID: MGI:5304754
Synonyms: shRNA-B
Gene: Col1a1  Location: Chr11:94936224-94953042 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Germline Transmission:  Earliest citation of germline transmission: J:179085
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Inducible, Inserted expressed sequence, Knockdown)
Mutation:    Insertion
  Col1a1tm1(tetO-RNAi:Rps19)Karl involves 1 genes/genome features (Rps19) View all
Mutation detailsRMCE on KH2 cells inserts an shRNA targeting Rsp19 behind a tetracycline responsive element and CMV promoter replacing the Pou5f1 cassette in Col1a1tm2(tetO-Pou5f1)Jae into the 3'UTR. The level of knock down by this allele following doxycycline treatment is lower compared to mice expressing Col1a1tm2(tetO-RNAi:Rps19)Karl . (J:179085)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  106 strains or lines available
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm2(tetO-Pou5f1)Jae.
Original:  J:179085 Jaako P, et al., Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia. Blood. 2011 Dec 1;118(23):6087-96
All:  2 reference(s)

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MGI 6.11
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