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Wisp3tm1(cre)Mawa
Targeted Allele Detail
Nomenclature
Symbol: Wisp3tm1(cre)Mawa
Name: WNT1 inducible signaling pathway protein 3; targeted mutation 1, Matthew L Warman
MGI ID: MGI:5302829
Synonyms: Wisp3GFP-Cre, Wisp3tm1(EGFP/cre)Mawa
Gene: Wisp3  Location: Chr10:39150971-39163794 bp, - strand  Genetic Position: Chr10, 20.19 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:179652
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsAn EGFP-IRES-cre-construct was introduced to the first exon of Wisp3, retaining the gene's ATG translation initiation site. No full-length Wisp3 transcripts can be detected by RT-PCR. Donating investigator indicates that expression of EGFP (enhanced green fluorescence protein) cannot be visualized by fluorescence microscopy. Hetero- and homozygous mice show no apparent phenotype. When males mutants are crossed with animals carrying a conditional (floxed) allele, cre-mediated recombination is observed in 100% of offspring. (Females occasionally also express cre in germ cells but at the precise stage is unclear. Cre-mediated recombination is observed in 7% of offspring from females.)There is little or no expression of cre in most other tissues examined, including muscle, kidney, bone, liver, and lung. Occasional recombination in brain, cartilage, and heart is seen. (J:179652, J:201718)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Wisp3     Summary of all recombinase alleles driven by Wisp3.
 

Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Wisp3 Mutation:  2 strains or lines available
References
Original:  J:179652 Warman ML, Generation of a knock-in cre recombinase allele of Wisp3. MGI Direct Data Submission. 2012;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/30/2015
MGI 5.22
The Jackson Laboratory