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Targeted Allele Detail
Symbol: Nkx2-1tm1.1(cre/ERT2)Zjh
Name: NK2 homeobox 1; targeted mutation 1.1, Z Josh Huang
MGI ID: MGI:5302534
Synonyms: Nkx2.1-CreER
Gene: Nkx2-1  Location: Chr12:56578743-56583693 bp, - strand  Genetic Position: Chr12, 24.42 cM, cytoband C1-C3
Germline Transmission:  Earliest citation of germline transmission: J:151755
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Project Collection: Neuroscience Blueprint cre
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
Mutation detailsA targeting vector was designed to insert a CreERT2 fusion gene , an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the NK2 homeobox 1 locus (Nkx2-1). This construct was electroporated into embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. These Nkx2.1-CreER (or Nkx2.1-CreERT2) mice were subsequently bred to C57BL/6 inbred mice for a few additional generations (and the FLPe transgene was removed). (J:151755)
Driver: Nkx2-1     Summary of all recombinase alleles driven by Nkx2-1. Comparative matrix view of recombinase activities

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Nkx2-1 Mutation:  5 strains or lines available
Original:  J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009;
All:  27 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory