Kiss1tm1.1(cre/EGFP)Stei
Targeted Allele Detail
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Symbol: |
Kiss1tm1.1(cre/EGFP)Stei |
Name: |
KiSS-1 metastasis-suppressor; targeted mutation 1.1, Robert A Steiner |
MGI ID: |
MGI:5298495 |
Synonyms: |
Kiss1Cre:Gfp, Kiss1-CreGFP, Kiss1Cre(v1) |
Gene: |
Kiss1 Location: Chr1:133254950-133257460 bp, + strand Genetic Position: Chr1, 57.91 cM
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Alliance: |
Kiss1tm1.1(cre/EGFP)Stei page
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Allele Type: |
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Targeted (Null/knockout, Recombinase, Reporter) |
Mutation: |
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Insertion
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Kiss1tm1.1(cre/EGFP)Stei expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A targeting vector was designed to insert a CreGFP fusion protein cassette and frt-flanked neomycin (neo) selection cassette upstream of the 5' translation start of the KiSS-1 metastasis-suppressor (Kiss1) targeted gene. Specifically, this targeting vector contained an enhanced green fluorescent protein (EGFP) and a Cre recombinase. This construct was electroporated into F1 hybrid (129S6/SvEvTac x C57BL/6NCr)-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. These Kiss1-CreGFP mice were bred with FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. When Kiss1 is induced, EGFP immunofluorescence is observed in the hypothalamic arcuate nucleus (ARC) of both sexes and anteroventral periventricular nucleus (AVPV) in females. Homozygotes are often infertile, but heterozygotes display normal fertility.
(J:178519, J:178520)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Kiss1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:178520 Navarro VM, et al., Regulation of NKB pathways and their roles in the control of Kiss1 neurons in the arcuate nucleus of the male mouse. Endocrinology. 2011 Nov;152(11):4265-75 |
All: |
22 reference(s) |
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