Kiss1^{tm1.1(cre/EGFP)Stei}
Targeted Allele Detail

Nomenclature

• Symbol: Kiss1^{tm1.1(cre/EGFP)Stei}
• Name: KiSS-1 metastasis-suppressor; targeted mutation 1.1, Robert A Steiner
• MGI ID: MGI:5298495
• Synonyms: Kiss1-CreGFP
• Gene: Kiss1 Location: Chr1:133321887-133329722 bp, + strand Genetic Position: Chr1, 57.91 cM

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178520
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a CreGFP fusion protein cassette and frt-flanked neomycin (neo) selection cassette upstream of the 5' translation start of the KiSS-1 metastasis-suppressor (Kiss1) targeted gene. Specifically, this targeting vector contained an enhanced green fluorescent protein (EGFP) and a Cre recombinase. This construct was electroporated into F1 hybrid (129S6/SvEvTac x C57BL/6NCr)-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. These Kiss1-CreGFP mice were bred with FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. When Kiss1 is induced, EGFP immunofluorescence is observed in the hypothalamic arcuate nucleus (ARC) of both sexes and anteroventral periventricular nucleus (AVPV) in females. Homozygotes are often infertile, but heterozygotes display normal fertility. (J:178519, J:178520)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Kiss1     Summary of all recombinase alleles driven by Kiss1.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kiss1 Mutation: 19 strains or lines available

References

• Original: J:178520 Navarro VM, et al., Regulation of NKB pathways and their roles in the control of Kiss1 neurons in the arcuate nucleus of the male mouse. Endocrinology. 2011 Nov;152(11):4265-75
• All: 16 reference(s)