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Kiss1tm1.1(cre/EGFP)Stei
Targeted Allele Detail
Nomenclature
Symbol: Kiss1tm1.1(cre/EGFP)Stei
Name: KiSS-1 metastasis-suppressor; targeted mutation 1.1, Robert A Steiner
MGI ID: MGI:5298495
Synonyms: Kiss1-CreGFP
Gene: Kiss1  Location: Chr1:133321887-133329722 bp, + strand  Genetic Position: Chr1, 57.91 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:178520
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Null/knockout, Recombinase, Reporter)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed to insert a CreGFP fusion protein cassette and frt-flanked neomycin (neo) selection cassette upstream of the 5' translation start of the KiSS-1 metastasis-suppressor (Kiss1) targeted gene. Specifically, this targeting vector contained an enhanced green fluorescent protein (EGFP) and a Cre recombinase. This construct was electroporated into F1 hybrid (129S6/SvEvTac x C57BL/6NCr)-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. These Kiss1-CreGFP mice were bred with FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. When Kiss1 is induced, EGFP immunofluorescence is observed in the hypothalamic arcuate nucleus (ARC) of both sexes and anteroventral periventricular nucleus (AVPV) in females. Homozygotes are often infertile, but heterozygotes display normal fertility. (J:178519, J:178520)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Kiss1     Summary of all recombinase alleles driven by Kiss1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Kiss1 Mutation:  19 strains or lines available
References
Original:  J:178520 Navarro VM, et al., Regulation of NKB pathways and their roles in the control of Kiss1 neurons in the arcuate nucleus of the male mouse. Endocrinology. 2011 Nov;152(11):4265-75
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/30/2021
MGI 6.16
The Jackson Laboratory