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Targeted Allele Detail
Symbol: Bhmttm1.2Zei
Name: betaine-homocysteine methyltransferase; targeted mutation 1.2, Steven Zeisel
MGI ID: MGI:5297083
Gene: Bhmt  Location: Chr13:93616891-93637961 bp, - strand  Genetic Position: Chr13, 47.87 cM, cytoband D1
Germline Transmission:  Earliest citation of germline transmission: J:177569
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsOne loxAPA site was inserted in intron 5 240 bp upstream of exon 6 and an FRT flanked MC1-neomycin cassette followed by a second loxP site was placed 330 bp from exon 7. The neomycin selection cassette was excised by crossing to Tg(ACTFLPe)9205Dym mice. Exons 6 and 7, containing the essential zinc binding domain, were then removed by crossing to Tg(EIIa-cre)C5379Lmgd mice. (J:177569)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Bhmt Mutation:  15 strains or lines available
Original:  J:177569 Teng YW, et al., Deletion of betaine-homocysteine s-methyltransferase in mice perturbs choline and 1-carbon metabolism, resulting in Fatty liver and hepatocellular carcinomas. J Biol Chem. 2011 Oct 21;286(42):36258-67
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
The Jackson Laboratory