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Targeted Allele Detail
Symbol: Col1a1tm3(tetO-Mirlet7g/Mir21)Gqda
Name: collagen, type I, alpha 1; targeted mutation 3, George Q Daley
MGI ID: MGI:5294613
Synonyms: iLet-7, let-7, let-7S21L
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Germline Transmission:  Earliest citation of germline transmission: J:177113
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
Mutation:    Insertion
Col1a1tm3(tetO-Mirlet7g/Mir21)Gqda expresses 2 genes
Mutation detailsRMCE on KH2 cells inserted a cassette that contains a splice acceptor, 2 polyadenylation sequence, tetracycline operator, a fusion of the Mirlet7g stem sequence and the Mir21 loop sequence, and an SV40 polyadenylation signal into the 3'UTR. No expression of the inserted sequence is detected in the absence of doxycycline treatment. (J:177113)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  141 strains or lines available
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae.
Original:  J:177113 Zhu H, et al., The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell. 2011 Sep 30;147(1):81-94
All:  17 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory