About   Help   FAQ
Col1a1tm2(tetO-LIN28B)Gqda
Targeted Allele Detail
Nomenclature
Symbol: Col1a1tm2(tetO-LIN28B)Gqda
Name: collagen, type I, alpha 1; targeted mutation 2, George Q Daley
MGI ID: MGI:5294612
Synonyms: iLIN28B
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:177113
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Humanized sequence, Inducible, Inserted expressed sequence, RMCE-ready)
Mutation:    Insertion
 
Col1a1tm2(tetO-LIN28B)Gqda expresses 1 gene
 
Mutation detailsRMCE on KH2 cells inserted a construct, which contains (5' to 3') a splice acceptor, 2 polyadenylation sequence, tetracycline operator, a flag-tagged human LIN28B cDNA, and an SV40 polyadenylation signal, into the 3'UTR. No expression of the inserted gene is detected in the absence of doxycycline treatment. (J:177113)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Loading...
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  141 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae.
References
Original:  J:177113 Zhu H, et al., The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell. 2011 Sep 30;147(1):81-94
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
11/23/2021
MGI 6.17
The Jackson Laboratory