Pds5atm1a(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Pds5atm1a(EUCOMM)Hmgu |
Name: |
PDS5 cohesin associated factor A; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5050206 |
Gene: |
Pds5a Location: Chr5:65763062-65855579 bp, - strand Genetic Position: Chr5, 33.76 cM, cytoband C3.3
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IMPC: |
Pds5a gene page |
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Mutant Cell Lines: |
HEPD0739_8_A03, HEPD0739_8_B03, HEPD0739_8_C03, HEPD0739_8_D01, HEPD0739_8_D04, HEPD0739_8_E01, HEPD0739_8_E02, HEPD0739_8_E03, HEPD0739_8_F01, HEPD0739_8_F04, HEPD0739_8_G01, HEPD0739_8_G03, HEPD0739_8_G04, HEPD0739_8_H02, HEPD0739_8_H03 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Pgk_P
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Mutation details: The L1L2_Pgk_P cassette was inserted at position 65816691 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 65817561. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
1 reference(s) |
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