Gt(ROSA)26Sor^tm2Thl
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm2Thl
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Thomas Ludwig
• MGI ID: MGI:5008628
• Synonyms: ROSA26-loxP-STOP-loxP-luciferase, stop-flox luciferase
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm2Thl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:172585
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The firefly luciferase gene (from the pGL2 plasmid, Promega) was introduced into the ROSA26 locus using a knock-in strategy. The targeting vector was constructed to contain the coding sequence of firefly luciferase downstream of a loxP-flanked STOP cassette (consisting of a promoter-less neomycin gene followed by a three SV40 polyadenylation sequences).

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:172585 Lei L, et al., Glioblastoma Models Reveal the Connection between Adult Glial Progenitors and the Proneural Phenotype. PLoS One. 2011;6(5):e20041
• All: 2 reference(s)