Tg(Nr4a1-EGFP/cre)820Khog
Transgene Detail

Summary

• Symbol: Tg(Nr4a1-EGFP/cre)820Khog
• Name: transgene insertion 820, Kristin A Hogquist
• MGI ID: MGI:5007644
• Synonyms: B6-820, Nur77^GFP, Nur77-GFPCre B6-820
• Transgene: Tg(Nr4a1-EGFP/cre)820Khog Location: unknown
• Alliance: Tg(Nr4a1-EGFP/cre)820Khog page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Nr4a1-EGFP/cre)820Khog expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nr4a1 (MGI:1352454)

• Mutation details: The 170 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP24-366J14, containing the entire Nr4a1 locus (Nur77) was obtained. An eGFP-hCre fusion protein cDNA sequence (enhanced green fluorescent protein fused to a codon-optimized "humanized" Cre to improve translational efficiency in eukaryotic cells), a frt-flanked neo cassette, and a TGA STOP codon was inserted into the ATG start site of the Nr4a1 gene on the RP24-366J14 BAC via homologous recombination/BAC recombineering. The frt-flanked neo was removed via arabinose treatment during BAC recombineering. A ~135 kb fragment from the modified BAC harboring the targeted Nr4a1 locus as well as 60 kb sequence flanking each side of the Nr4a1 locus (including the Acvr1b, A330009N23Rik, Grasp, 9430023L20Rik and 6030408B16Rik loci) was purified and then microinjected into C57BL/6J embryos. The resulting Nur77GFP BAC transgenic founder mice were bred with C57BL/6NCr mice. Transgenic mice from the B6-820 founder line were found with high GFP expression levels in myeloid lineage cells from spleen (but not lymph node), as well as low GFP expression levels in T and B lymphocytes. (J:172415)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nr4a1 (mouse) Summary of all recombinase alleles driven by Nr4a1. Alliance mouse Nr4a1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:172415 Moran AE, et al., T cell receptor signal strength in Treg and iNKT cell development demonstrated by a novel fluorescent reporter mouse. J Exp Med. 2011 Jun 6;208(6):1279-89
• All: 132 reference(s)