Orc3^tm1.1Zhua
Targeted Allele Detail

Summary

• Symbol: Orc3^tm1.1Zhua
• Name: origin recognition complex, subunit 3; targeted mutation 1.1, Zhen Huang
• MGI ID: MGI:4947979
• Gene: Orc3 Location: Chr4:34570796-34614944 bp, - strand Genetic Position: Chr4, 16.81 cM, cytoband A3
• Alliance: Orc3^tm1.1Zhua page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:170581
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An FRT flanked neo cassette with a 5' loxP site was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 7. Flp-mediated recombination removed the neo cassette leaving exons 5 through 7 floxed. An 84% reduction in protein expression was confirmed by western blot analysis on E13.5 brain extracts (J:170581)

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Orc3 Mutation: 47 strains or lines available

References

• Original: J:170581 Kwon HJ, et al., Radial glia regulate Cajal-Retzius cell positioning in the early embryonic cerebral cortex. Dev Biol. 2011 Mar 1;351(1):25-34
• All: 1 reference(s)