Pld6^tm1.1Hsas
Targeted Allele Detail

Summary

• Symbol: Pld6^tm1.1Hsas
• Name: phospholipase D family member 6; targeted mutation 1.1, Hiroyuki Sasaki
• MGI ID: MGI:4946932
• Gene: Pld6 Location: Chr11:59674723-59678471 bp, - strand Genetic Position: Chr11, 37.79 cM
• Alliance: Pld6^tm1.1Hsas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169934
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment including all of exon 1 was replaced with a floxed neo cassette via homologous recombination. Cre mediated recombination then removed the neo cassette. Western blot analysis confirmed the absence of protein expression in testes from homozygous mice. (J:169934)

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pld6 Mutation: 9 strains or lines available

References

• Original: J:169934 Watanabe T, et al., MITOPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline. Dev Cell. 2011 Mar 15;20(3):364-75
• All: 4 reference(s)