Bmp4^{tm2(tetO-Bmp4,lacZ)Jfm}
Targeted Allele Detail

Summary

• Symbol: Bmp4^{tm2(tetO-Bmp4,lacZ)Jfm}
• Name: bone morphogenetic protein 4; targeted mutation 2, James F Martin
• MGI ID: MGI:4941211
• Synonyms: Bmp4^tetO
• Gene: Bmp4 Location: Chr14:46620982-46628126 bp, - strand Genetic Position: Chr14, 23.95 cM
• Alliance: Bmp4^{tm2(tetO-Bmp4,lacZ)Jfm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169047
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Hypomorph, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Bmp4^{tm2(tetO-Bmp4,lacZ)Jfm} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Bmp4 (MGI:88180)

• Bmp4^{tm2(tetO-Bmp4,lacZ)Jfm} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: The insertion of a construct consisting of a tetracycline operator, CMV promoter, Bmp4 cDNA, IRES lacZ, and FRT flanked pgk-neo cassette, and a poly-adenylation sequence was inserted in place of a 665 bp region that includes the basal promoter and exon1 of the gene. Analysis of p-Smad1/5/8 activity in heterozygous mice indicates this is a hypomorphic allele. (J:169047, J:181229)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bmp4 Mutation: 21 strains or lines available

References

• Original: J:169047 Wang J, et al., Bmp signaling regulates myocardial differentiation from cardiac progenitors through a MicroRNA-mediated mechanism. Dev Cell. 2010 Dec 14;19(6):903-12
• All: 3 reference(s)