Kif20b^magoo
Chemically induced Allele Detail

Summary

• Symbol: Kif20b^magoo
• Name: kinesin family member 20B; magoo
• MGI ID: MGI:4888744
• Synonyms: ND15
• Gene: Kif20b Location: Chr19:34899761-34953145 bp, + strand Genetic Position: Chr19, 29.79 cM
• Alliance: Kif20b^magoo page

Mutation origin

• Strain of Origin: BALB/c x C57BL/6

Mutation description

• Allele Type: Chemically induced (ENU) (Null/knockout)
• Mutation: Single point mutation
• Mutation details: ENU induced a T-to-G mutation 13 bases upstream of exon 19. RT-PCR and sequencing analysis showed the inclusion of 29 bases of intronic sequence in the mutant transcript, owing to the use of an alternative the acceptor site. The inclusion of the additional bases should cause a frameshift mutation and multiple premature stop codons. Semi-quantitative RT-PCR analysis showed that the mutant mRNA was less abundant compared to wild-type mRNA. Immunoblot analysis failed to detect protein in brain lysates from homozygous mutants. (J:205043)

Disease models

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kif20b Mutation: 69 strains or lines available

Notes

Originally generated from male mice expressing Tg(Mbp-MAPT/lacZ)#Caw.

References

• Original: J:168258 Dwyer ND, et al., A forward genetic screen with a thalamocortical axon reporter mouse yields novel neurodevelopment mutants and a distinct emx2 mutant phenotype. Neural Dev. 2011;6:3
• All: 5 reference(s)