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Transgene Detail
Symbol: Tg(Pmch-cre)1Lowl
Name: transgene insertion 1, Bradford B Lowell
MGI ID: MGI:4887235
Synonyms: MCH-Cre
Transgene: Tg(Pmch-cre)1Lowl  Location: unknown  
Strain of Origin:  FVB/NJ
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
Mutation detailsHomologous recombination with EL250 cells was induced to insert a cre-polyA-FRT-Neo-FRT cassette into a BAC containing the Pmch (pro-melanin-concentrating hormone; MCH) at the Pmch translational start site with concomitant replacement of the 26 nucleotides following it. Arabinose-induced Flp recombination in bacterial cells removed the kanamycin cassette. The purified, linearized construct was injected into the pronuclei of FVB one-cell embryos. Eight lines were generated and crossed to Z/EG reporter animals. 2 lines exhibited GFP expression in the lateral hypothalamus and zona incerta, known sites of endogenous Pmch expression. Cre expression in one of these lines of characterized further. (J:167906)
 Tissue activity of this recombinase allele
Driver: Pmch     Summary of all recombinase alleles driven by Pmch.

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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:167906 Kong D, et al., Glucose stimulation of hypothalamic MCH neurons involves K(ATP) channels, is modulated by UCP2, and regulates peripheral glucose homeostasis. Cell Metab. 2010 Nov 3;12(5):545-52
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 5.21
The Jackson Laboratory