Hes1^tm1.1Frad
Targeted Allele Detail

Summary

• Symbol: Hes1^tm1.1Frad
• Name: hes family bHLH transcription factor 1; targeted mutation 1.1, Freddy Radtke
• MGI ID: MGI:4868726
• Synonyms: Hes1^lox
• Gene: Hes1 Location: Chr16:29883259-29886614 bp, + strand Genetic Position: Chr16, 21.09 cM
• Alliance: Hes1^tm1.1Frad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167000
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Tow loxP sites were inserted to flank part of intron 1, exons 2-4 and the 3'UTR. An FRT site-flanked neomycin resistance gene was introduced followed by the second loxP site, and removed later by Flp-mediated recombination. A beta-galactosidase gene was also inserted after the second loxP site. Cre-mediated recombination of the loxP-flanked gene segment creates an in frame translation of beta-galactosidase. (J:167000)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hes1 Mutation: 21 strains or lines available

References

• Original: J:167000 Wendorff AA, et al., Hes1 is a critical but context-dependent mediator of canonical Notch signaling in lymphocyte development and transformation. Immunity. 2010 Nov 24;33(5):671-84
• All: 1 reference(s)