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Tg(Thy1-Clomeleon)12Gjau
Transgene Detail
Nomenclature
Symbol: Tg(Thy1-Clomeleon)12Gjau
Name: transgene insertion 12, George Augustine
MGI ID: MGI:4847925
Synonyms: Tg(Thy1-CFP,Topaz)CLM12Gjau, Tg(Thy1-CFP,YFP)CLM12Gjau
Transgene: Tg(Thy1-Clomeleon)12Gjau  Location: unknown  
Transgene
origin
Strain of Origin:  ICR
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsA transgenic construct containing Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2) was injected into fertilized ICR mouse eggs. Founder line CLM12 was subsequently established. Transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP that acts as a ratiometric indicator for chloride ions, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2). Transgene expression in founder line CLM12 is detected at high levels in the thalamus, cerebellar nuclei. No expression is detected in layers 1 and 2 of the neocortex, hippocampal regions CA2 and CA3, cerebellar purkinje cells, or retinal ganglion cells. In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer) acceptor for CFP. As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases. Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-]. (J:166511)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:165251 Duebel J, et al., Two-photon imaging reveals somatodendritic chloride gradient in retinal ON-type bipolar cells expressing the biosensor Clomeleon. Neuron. 2006 Jan 5;49(1):81-94
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory