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Targeted Allele Detail
Symbol: Hand1tm2.1(Hand1)Abfi
Name: heart and neural crest derivatives expressed 1; targeted mutation 2.1, Anthony B Firulli
MGI ID: MGI:4843975
Synonyms: Hand1deltaNeo, Hand1tm1.1(Hand1)Abfi
Gene: Hand1  Location: Chr11:57828713-57832147 bp, - strand  Genetic Position: Chr11, 35.27 cM
Germline Transmission:  Earliest citation of germline transmission: J:164028
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Allele Type:    Targeted (Hypomorph)
Mutation:    Insertion
Mutation detailsThe coding region of Hand1 from a 600-bp cDNA initiating at the ATG and terminating at the stop codon followed by an EGFP expression cassette and a PGKNeo cassette flanked by loxP sites were inserted in frame at the position of the initiation codon. The PGKNeo cassette from Hand1tm2(Hand1)Abfi was removed via cre-mediated recombination. EGFP expression is not detected in E9.5 embryos. qRT-PCR indicates that heterozygotes express about 50% of wild-type mRNA levels while homozygotes express at 30% that of wild-type, indicating that this is a hypomorphic allele. In situ analysis indicates that expression is not uniformly reduced throughout the embryo. (J:164028)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 6 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hand1 Mutation:  3 strains or lines available
Original:  J:164028 Firulli BA, et al., Analysis of a Hand1 hypomorphic allele reveals a critical threshold for embryonic viability. Dev Dyn. 2010 Oct;239(10):2748-60
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory