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Targeted Allele Detail
Symbol: Pou2f1tm1Cav
Name: POU domain, class 2, transcription factor 1; targeted mutation 1, Fatima Cavaleri
MGI ID: MGI:4838111
Gene: Pou2f1  Location: Chr1:165865154-166002678 bp, - strand  Genetic Position: Chr1, 73.21 cM
Germline Transmission:  Earliest citation of germline transmission: J:165572
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsMost of intron 10 and all of exon 11, encoding the linker domain and 5' terminus of the POUh domain, were replaced with a neo cassette via homologous recombination. RT-PCR detected the presence of a mutant transcript with exon 10 spliced to exon 12. EMSA analysis with nuclear extracts from spleen and thymus cells from heterozygous mice indicates that any truncated protein made is not able to bind DNA. This suggests that this mutation results in a null allele. (J:165572)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pou2f1 Mutation:  40 strains or lines available
Original:  J:165572 Sebastiano V, et al., Oct1 regulates trophoblast development during early mouse embryogenesis. Development. 2010 Nov;137(21):3551-60
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.15
The Jackson Laboratory