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Mir33tm1.1Koho
Targeted Allele Detail
Nomenclature
Symbol: Mir33tm1.1Koho
Name: microRNA 33; targeted mutation 1.1, Koh Ono
MGI ID: MGI:4837623
Gene: Mir33  Location: Chr15:82082323-82082391 bp, + strand  Genetic Position: Chr15, 38.54 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:165420
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA floxed pgk-neo cassette replaced the pre microRNA sequence located in intron 16 of Srebf2. Cre mediated recombination removed the neo cassette. Western blot and RT-PCR analysis confirmed that deletion of the microRNA did not disrupt splicing or expression of Srebf2. (J:165420)
Generation of the Mir33tm1.1Koho allele
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mir33 Mutation:  1 strain or line available
References
Original:  J:165420 Horie T, et al., MicroRNA-33 encoded by an intron of sterol regulatory element-binding protein 2 (Srebp2) regulates HDL in vivo. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17321-6
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/10/2022
MGI 6.19
The Jackson Laboratory