Tlr8^tm1Vlcg
Targeted Allele Detail

Summary

• Symbol: Tlr8^tm1Vlcg
• Name: toll-like receptor 8; targeted mutation 1, Velocigene
• MGI ID: MGI:4837530
• Synonyms: VG230
• Gene: Tlr8 Location: ChrX:166025692-166047325 bp, - strand Genetic Position: ChrX, 78.29 cM, cytoband F5
• Alliance: Tlr8^tm1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165267
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 2.3 kb of the coding sequence (amino acids 103-881) was replaced with a lacZ/neo cassette. RT-PCR and northern blot analysis confirmed the absence of transcript expression in unstimulated and LPS- or R848-stimulated bone marrow-derived dendritic cells. Beta galactose staining showed expression of lacZ in mutant lymph nodes and spleens. (J:165267)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tlr8 Mutation: 11 strains or lines available

References

• Original: J:102090 Valenzuela DM, et al., High-throughput engineering of the mouse genome coupled with high-resolution expression analysis. Nat Biotechnol. 2003 Jun;21(6):652-9
• All: 8 reference(s)