About   Help   FAQ
Tg(Car1-cre)5Flt
Transgene Detail
Nomenclature
Symbol: Tg(Car1-cre)5Flt
Name: transgene insertion 5, James C Fleet
MGI ID: MGI:4835007
Synonyms: CAC5
Transgene: Tg(Car1-cre)5Flt  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6J
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
 
Mutation detailsThe 10.6 kb colon-specific promoter and the share 2.5 kb erythroid/colon enhancer from the mouse Car1 gene were PCR amplified and cloned in front of the cre recombinase gene in a plasmid vector. The purified construct was used to generated transgenic mice. Two lines were selected for further analysis. Line 5 (CAC5 with 40 copies to the transgene insertion) was shown by PCR to have 10-fold higher transgene mRNA levels than line 6 (CAC6 having 20 copies). The mRNA was detectable only in the large intestine from cecum to distal colon. A low level of mRNA expression was detected in the liver, but no other tissues analyzed expressed the transgene. (J:164247)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Car1     Summary of all recombinase alleles driven by Car1. Comparative matrix view of recombinase activities
 

Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:164247 Xue Y, et al., Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestine. Mol Cancer Res. 2010 Aug;8(8):1095-104
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
03/30/2021
MGI 6.16
The Jackson Laboratory