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Targeted Allele Detail
Symbol: Nfkbiatm1.1Pjc
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha; targeted mutation 1.1, Paul J Chiao
MGI ID: MGI:4829878
Synonyms: IkappaBalphaM, IkBaM
Gene: Nfkbia  Location: Chr12:55489411-55492647 bp, - strand  Genetic Position: Chr12, 24.0 cM, cytoband C1-C3
Germline Transmission:  Earliest citation of germline transmission: J:163686
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Allele Type:    Targeted
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsTwo well defined NFKB binding sites (M1 and M2) and 4 NFKB like binding sites (M3 - M6) in the promoter region were mutated, a FLAG tag was attached to exon 1, and a floxed neo cassette was inserted in intron 1 via homologous recombination. Cre mediated recombination removed the neo cassette. The locations of and mutations in the 6 binding sites are as follows: -115 - -93, a TCC motif changed to a CCG (M1); -143 - -122, a CCC motif changed to an AGT (M2); -227 - -205, a TCT motif changed to a CAC (M3); -292 - -271, a CC motif changed to a AA (M4); -319 - -297, a GGG motif changed to a TTT (M5); -387 - -365, a CCC motif changed to a GGG (M6). Chromatin immunoprecipitation analysis confirmed the loss of kappa B enhancer function in MEFs from homozygous mice. (J:163686)
View phenotypes for all genotypes (concatenated display).
Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nfkbia Mutation:  10 strains or lines available
Original:  J:163686 Peng B, et al., Defective feedback regulation of NF-kappaB underlies Sjogren's syndrome in mice with mutated kappaB enhancers of the IkappaBalpha promoter. Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):15193-8
All:  1 reference(s)

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MGI 6.03
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