Jak2^tm1.2Ble
Targeted Allele Detail

Summary

• Symbol: Jak2^tm1.2Ble
• Name: Janus kinase 2; targeted mutation 1.2, Benjamin L Ebert
• MGI ID: MGI:4818801
• Synonyms: Jak2V617F, Jak2^VF
• Gene: Jak2 Location: Chr19:29229228-29290480 bp, + strand Genetic Position: Chr19, 23.73 cM
• Alliance: Jak2^tm1.2Ble page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160883
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Intragenic deletion, Single point mutation
• Mutation details: A loxP site was inserted upstream of exon 14 and a cassette, consisting of a loxP511 site, a linker, an inverted loxP site, an inverted exon 14 containing a G1849T mutation, an inverted loxP511 site, and an FRT flanked neo selection cassette, was inserted downstream of exon 14 via homologous recombination. The neo cassette was removed by flp mediated recombination. Cre mediated recombination results in the removal of the endogenous exon 14 and the inversion of the mutated exon 14. The G1849T mutation results in a V617F substitution. (J:160883)

Disease models

Expression

In Mice Carrying this Mutation:	3 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Jak2 Mutation: 57 strains or lines available

References

• Original: J:160883 Mullally A, et al., Physiological Jak2V617F expression causes a lethal myeloproliferative neoplasm with differential effects on hematopoietic stem and progenitor cells. Cancer Cell. 2010 Jun 15;17(6):584-96
• All: 32 reference(s)