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Targeted Allele Detail
Symbol: Actbtm3.1(Sirt1)Npa
Name: actin, beta; targeted mutation 3.1, Novartis Pharma AG
MGI ID: MGI:4456082
Synonyms: SIRT1-KI+
Gene: Actb  Location: Chr5:142903115-142906754 bp, - strand  Genetic Position: Chr5, 81.8 cM
Germline Transmission:  Earliest citation of germline transmission: J:160212
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6J
Allele Type:    Targeted (Inserted expressed sequence)
Mutation:    Insertion
Actbtm3.1(Sirt1)Npa expresses 1 gene
Mutation detailsThe 3' UTR was replaced with a cassette containing an IRES sequence, floxed neo cassette, mouse Sirt1 cDNA, and a bovine growth hormone polyadenylation sequence. Cre mediated recombination removed the neo cassette. Western blot analysis confirmed expression of the cDNA in white adipose tissue, brown adipose tissue, brain, calvaria cells of the skull, and mouse embryonic fibroblasts but not in the liver or muscle. Normal expression of Actb was confirmed by western blot analysis. (J:160212)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Actb Mutation:  32 strains or lines available
Original:  J:160212 Bordone L, et al., SIRT1 transgenic mice show phenotypes resembling calorie restriction. Aging Cell. 2007 Dec;6(6):759-67
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory