Slc22a2^tm2Ahs
Targeted Allele Detail

Summary

• Symbol: Slc22a2^tm2Ahs
• Name: solute carrier family 22 (organic cation transporter), member 2; solute carrier family 22 (organic cation transporter), member 2; targeted mutation 2, Alfred H Schinkel
• MGI ID: MGI:4454788
• Synonyms: Oct1/2^-
• Gene: Slc22a2 Location: Chr17:12803076-12847376 bp, + strand Genetic Position: Chr17, 8.61 cM
• Alliance: Slc22a2^tm2Ahs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86270
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A modified E14 ES cell line heterozygous for the Slc22a1^tm1Ahs allele was targeted a second time. Exon 1 (encoding the start codon, the putative TMD1 domain, and extracellular loop between the putative TMD1 and TMD2 domains), as well as upstream sequence were replaced with a neomycin selection cassette inserted by homologous recombination. Absence of Slc22a2 mRNA in double homozygous mutant mice was confirmed by Northern blotting and RNase protection analysis. Western analysis verified that no Slc22a1 protein was detectable in livers and kidneys of double mutant mice. (J:86270)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc22a2 Mutation: 39 strains or lines available

References

• Original: J:86270 Jonker JW, et al., Deficiency in the organic cation transporters 1 and 2 (Oct1/Oct2 [Slc22a1/Slc22a2]) in mice abolishes renal secretion of organic cations. Mol Cell Biol. 2003 Nov;23(21):7902-8
• All: 4 reference(s)