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Slc22a2tm2Ahs
Targeted Allele Detail
Nomenclature
Symbol: Slc22a2tm2Ahs
Name: solute carrier family 22 (organic cation transporter), member 2; solute carrier family 22 (organic cation transporter), member 2; targeted mutation 2, Alfred H Schinkel
MGI ID: MGI:4454788
Synonyms: Oct1/2-
Gene: Slc22a2  Location: Chr17:12584189-12628465 bp, + strand  Genetic Position: Chr17, 8.61 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:86270
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA modified E14 ES cell line heterozygous for the Slc22a1tm1Ahs allele was targeted a second time. Exon 1 (encoding the start codon, the putative TMD1 domain, and extracellular loop between the putative TMD1 and TMD2 domains), as well as upstream sequence were replaced with a neomycin selection cassette inserted by homologous recombination. Absence of Slc22a2 mRNA in double homozygous mutant mice was confirmed by Northern blotting and RNase protection analysis. Western analysis verified that no Slc22a1 protein was detectable in livers and kidneys of double mutant mice. (J:86270)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Slc22a2 Mutation:  20 strains or lines available
References
Original:  J:86270 Jonker JW, et al., Deficiency in the organic cation transporters 1 and 2 (Oct1/Oct2 [Slc22a1/Slc22a2]) in mice abolishes renal secretion of organic cations. Mol Cell Biol. 2003 Nov;23(21):7902-8
All:  4 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory