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Targeted Allele Detail
Symbol: Chrna3tm1.1Hwrt
Name: cholinergic receptor, nicotinic, alpha polypeptide 3; targeted mutation 1.1, Edward Hawrot
MGI ID: MGI:4442209
Synonyms: alpha3[5] nAChR
Gene: Chrna3  Location: Chr9:54917401-54933846 bp, - strand  Genetic Position: Chr9, 29.84 cM
Germline Transmission:  Earliest citation of germline transmission: J:157611
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Allele Type:    Targeted (Not Specified)
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsA targeting construct was made containing a mutated exon 5 of mouse nicotinic acetylcholine receptor (nAChR) neuronal alpha3 subunit (alpha3[5]), and a loxP-flanked neomycin (neo) resistance cassette upstream of exon 5. This mutated alpha3[5] gene contained five altered nucleotides, Y184W, E187W, I188V, K189Y, and N191T, in the loop C region corresponding to residues from the alpha1 subunit of nAchR from Torpedo californica, the Pacific electric ray which conveys alpha-bungarotoxin (alphaBTX) sensitivity. Cre mediated recombination removed the neo cassette. (J:157611)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Chrna3 Mutation:  25 strains or lines available
Original:  J:157611 Caffery PM, et al., Engineering neuronal nicotinic acetylcholine receptors with functional sensitivity to alpha-bungarotoxin: a novel alpha3-knock-in mouse. Eur J Neurosci. 2009 Dec 3;30(11):2064-76
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory