Il7r^{tm1.1(icre)Hrr}
Targeted Allele Detail

Summary

• Symbol: Il7r^{tm1.1(icre)Hrr}
• Name: interleukin 7 receptor; targeted mutation 1.1, Hans-Reimer Rodewald
• MGI ID: MGI:4441349
• Synonyms: CD127-Cre-Tg, Il7r^Cre
• Gene: Il7r Location: Chr15:9505874-9530262 bp, - strand Genetic Position: Chr15, 4.16 cM
• Alliance: Il7r^{tm1.1(icre)Hrr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158903
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Il7r^{tm1.1(icre)Hrr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Il7r (MGI:96562)

• Mutation details: The targeting construct consisted, from 5' to 3', of a homology arm with sequence upstream of the Il7r ATG start site, a splice donor site, an intron and a splice acceptor site from the rabbit b-globin gene, the coding sequence of codon-improved cre (icre), a poly-adenylation signal from the rabbit b-globin gene, an FRT-flanked neomycin resistance gene, a 3' homology sequence and a diphtheria toxin subunit A negative selection sequence. Homologous recombination was carried out in E14.1 ES cells. To avoid potential impact of the Neo resistance gene with its enhancer and promoter sequences on iCre expression, the neo cassette was removed from the targeted locus by transient expression of Flp-recombinase, leaving only a single FRT-site in the genome. (J:158903)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Il7r (mouse) Summary of all recombinase alleles driven by Il7r. Alliance mouse Il7r gene page

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il7r Mutation: 29 strains or lines available

References

• Original: J:158903 Schlenner SM, et al., Fate mapping reveals separate origins of T cells and myeloid lineages in the thymus. Immunity. 2010 Mar 26;32(3):426-36
• All: 54 reference(s)