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Targeted Allele Detail
Symbol: Fgf8tm2.1Jyhl
Name: fibroblast growth factor 8; targeted mutation 2.1, James Y H Li
MGI ID: MGI:4437219
Synonyms: Fgf8deltaa
Gene: Fgf8  Location: Chr19:45736798-45742915 bp, - strand  Genetic Position: Chr19, 38.75 cM, cytoband C3-D
Germline Transmission:  Earliest citation of germline transmission: J:156717
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA point mutation into the 3' alternative splice acceptor of Fgf8 exon 1D, changing the sequence from cagCATGTG to caaCATGTG. A floxed neo selection cassette was place 400 bp downstream of exon 1D. The cassette was removed by cre mediated recombination. RT-PCR demonstrated that the transcripts of Fgf8a-containing spliceforms (a, c, e, and g) were missing, while Fgf8b-containing spliceforms, which utilize the remaining alternative splice acceptor of the exon 1D, are expressed. (J:156717)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Fgf8 Mutation:  10 strains or lines available
Original:  J:156717 Guo Q, et al., Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum. Dev Biol. 2010 Feb 15;338(2):183-92
All:  1 reference(s)

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MGI 6.12
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