Ctsz^tm1Thre
Targeted Allele Detail

Summary

• Symbol: Ctsz^tm1Thre
• Name: cathepsin Z; targeted mutation 1, Thomas Reinheckel
• MGI ID: MGI:4436876
• Synonyms: Ctsz^-
• Gene: Ctsz Location: Chr2:174269287-174280832 bp, - strand Genetic Position: Chr2, 97.94 cM
• Alliance: Ctsz^tm1Thre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157541
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 2, which contains the active site cysteine critical for enzyme activity, and part of intron 3 were deleted by homologous recombination and replaced by a cassette comprising an independent ribosomal entry sequence (IRES), followed by a lacZ reporter gene and a neomycin cassette. Disruption of the gene was confirmed by RT-PCR and Western blot analyses. (J:157541)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctsz Mutation: 9 strains or lines available

References

• Original: J:157541 Sevenich L, et al., Synergistic antitumor effects of combined cathepsin B and cathepsin Z deficiencies on breast cancer progression and metastasis in mice. Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2497-502
• All: 10 reference(s)