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Targeted Allele Detail
Symbol: Nubp1tm1a(EUCOMM)Wtsi
Name: nucleotide binding protein 1; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4431633
Gene: Nubp1  Location: Chr16:10411948-10424428 bp, + strand  Genetic Position: Chr16, 5.61 cM
Mutant Cell Lines:  EPD0089_1_A02, EPD0089_1_A03, EPD0089_1_B01, EPD0089_1_B02, EPD0089_1_B03, EPD0089_1_C01, EPD0089_1_C03, EPD0089_1_D01, EPD0089_1_E02, EPD0089_1_H03
Germline Transmission:  Earliest citation of germline transmission: J:191793
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt1
Mutation detailsThe L1L2_gt1 cassette was inserted at position 10413156 of Chromosome 16 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 10414011. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Nubp1 Mutation:  13 strains or lines available
Cell line EPD0089_1_C03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:191793
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory