Ffar4^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ffar4^{tm1(KOMP)Wtsi}
• Name: free fatty acid receptor 4; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4431463
• Gene: Ffar4 Location: Chr19:38085527-38102711 bp, + strand Genetic Position: Chr19, 32.73 cM
• IMPC: Ffar4 gene page

Mutation origin

• Mutant Cell Lines: EPD0447_3_A09, EPD0447_3_B11, EPD0447_3_C10, EPD0447_3_G10
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 213 starting at position 38095814 and ending at position 38096027 of Chromosome 19 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Ffar4 Mutation: 22 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)