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Targeted Allele Detail
Symbol: Epha4tm1.2Bzh
Name: Eph receptor A4; targeted mutation 1.2, Binhai Zheng
MGI ID: MGI:4430286
Synonyms: EphA4mCFPNeoNull
Gene: Epha4  Location: Chr1:77367185-77515088 bp, - strand  Genetic Position: Chr1, 39.55 cM, cytoband C1-C5
Germline Transmission:  Earliest citation of germline transmission: J:157159
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsTwo loxP sites were inserted flanking exon 3. Preceding the floxed exon 3, a reporter/selection cassette (SA-mCFP-Neo) flanked by two Frt sites, containing a splice acceptor, a membrane-targeted cyan fluorescent protein reporter gene and a neo cassette, was inserted. After Cre-mediated excision of exon 3, a frameshift in the downstream coding sequence resulted in a null allele and a functional mCFP reporter. Western blot analysis on total brain extracts and immunostaining on adult brain tissue confirmed the absence of the protein in homozygous mutants. Immunohistochemistry also confirmed mCFP expression in the hippocampus. (J:157159)
Generation of the Epha4tm1Bzh, Epha4tm1.1Bzh and Epha4tm1.2Bzh alleles
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Epha4 Mutation:  9 strains or lines available
Original:  J:157159 Herrmann JE, et al., Generation of an EphA4 conditional allele in mice. Genesis. 2010 Feb;48(2):101-5
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.15
The Jackson Laboratory