Kdr^tm1.1Eia
Targeted Allele Detail

Summary

• Symbol: Kdr^tm1.1Eia
• Name: kinase insert domain protein receptor; targeted mutation 1.1, Anne Eichmann
• MGI ID: MGI:4429911
• Synonyms: vegfr2^egfp
• Gene: Kdr Location: Chr5:76093487-76139118 bp, - strand Genetic Position: Chr5, 40.23 cM
• Alliance: Kdr^tm1.1Eia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156869
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An EGFP reporter followed by poly(A) signal and a loxP-flanked neomycin selection cassette were inserted into the initiation codon within exon 1 of the gene. The floxed selection cassette in the vector was excised by Cre-mediated recombination by transient transfection with a Cre-expressing plasmid in targeted ES cells. The embryos showed a typical vascular pattern of expression. Real-time PCR analysis of hearts isolated from 5 days old mice showed that the expression of the gene in heterozygous mice was reduced to about 50% of wild-type levels. (J:156869)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdr Mutation: 71 strains or lines available

References

• Original: J:156869 Xu Y, et al., Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3. J Cell Biol. 2010 Jan 11;188(1):115-30
• All: 2 reference(s)