Tg(Gjb6-cre/ERT2)53-33Fwp
Transgene Detail

Summary

• Symbol: Tg(Gjb6-cre/ERT2)53-33Fwp
• Name: transgene insertion 53-33, Frank W Pfrieger
• MGI ID: MGI:4420273
• Synonyms: Cx30-CreER^T2, Tg(Cx30-CreER^T2)53-33
• Transgene: Tg(Gjb6-cre/ERT2)53-33Fwp Location: unknown
• Alliance: Tg(Gjb6-cre/ERT2)53-33Fwp page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Gjb6-cre/ERT2)53-33Fwp expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gjb6 (MGI:107588)

• Mutation details: The creER^T2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Gjb6 gene located in BAC RP24-368M7. The linearized vector was injected into pronuclei of fertilized FVB/N eggs. Line 53-33 displayed high quantities of CreER^T2 transcripts in several brain regions including cortex, cerebellum, hippocampus, mesencephalon, and medulla oblongata. CreER^T2 and Cx30 mRNA were also present in the skin. (J:156087)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gjb6 (mouse) Summary of all recombinase alleles driven by Gjb6. Comparative matrix view of recombinase activities Alliance mouse Gjb6 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:156087 Slezak M, et al., Transgenic mice for conditional gene manipulation in astroglial cells. Glia. 2007 Nov 15;55(15):1565-76
• All: 15 reference(s)