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Targeted Allele Detail
Symbol: Gad2tm2(cre)Zjh
Name: glutamic acid decarboxylase 2; targeted mutation 2, Z Josh Huang
MGI ID: MGI:4418713
Synonyms: Gad2Cre, Gad2-iCre, Gad2-ires-Cre, GAD65-Cre, GAD65-iCre, GAD65-ires-Cre
Gene: Gad2  Location: Chr2:22512262-22583889 bp, + strand  Genetic Position: Chr2, 15.15 cM, cytoband A-B
Germline Transmission:  Earliest citation of germline transmission: J:151755
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Project Collection: Neuroscience Blueprint cre
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
Mutation detailsA targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the Gad2 (glutamic acid decarboxylase 2) gene. This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the selection cassette. The FLPe transgene was subsequently bred out of the background. (J:151755)
 Tissue activity of this recombinase allele
Driver: Gad2     Summary of all recombinase alleles driven by Gad2. Comparative matrix view of recombinase activities

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Gad2 Mutation:  47 strains or lines available
Original:  J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009;
All:  197 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory