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Tg(BEST1-rtTA,tetO-cre)1Yzl
Transgene Detail
Nomenclature
Symbol: Tg(BEST1-rtTA,tetO-cre)1Yzl
Name: transgene insertion 1, Yun-Zheng Le
MGI ID: MGI:4398912
Synonyms: PVMD2-rtTA::tetO-PhCMVcre, VMD2-cre
Transgene: Tg(BEST1-rtTA,tetO-cre)1Yzl  Location: unknown  
Transgene
origin
Strain of Origin:  FVB/N
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase, Transactivator)
Inducer:    doxycycline
Mutation:    Insertion
 
Mutation detailsTwo transgenic fragments were co-injected to generate the mouse line. One fragment contains a 3 kb human BEST1 promoter fragment driving expression of the tetracycline-inducible transactivator rtTA and the SV40 polyA sequence. The second fragment consists of the tetracycline-responsive regulatory element (tetO) and human cytomegalovirus immediate early enhancer (hCMV) controlling expression of a translationally optimized cre sequence followed by the mouse metallothionein (Mt1) polyadenylation signal. After co-injection into zygotes, 10 founders were obtained with co-integration of both fragments into a single chromosome being observed in all founders. One line demonstrating substantial activity in Muller glial cells was characterized further. (J:154799)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: BEST1     Summary of all recombinase alleles driven by BEST1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:132948 Le YZ, et al., Inducible expression of cre recombinase in the retinal pigmented epithelium. Invest Ophthalmol Vis Sci. 2008 Mar;49(3):1248-53
All:  32 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/12/2020
MGI 6.15
The Jackson Laboratory