Stx3^{Gt(EUCE320f12)Hmgu}
Gene trapped Allele Detail

Summary

• Symbol: Stx3^{Gt(EUCE320f12)Hmgu}
• Name: syntaxin 3; gene trap EUCE320f12, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4391716
• Gene: Stx3 Location: Chr19:11752482-11796767 bp, - strand Genetic Position: Chr19, 8.52 cM
• Alliance: Stx3^{Gt(EUCE320f12)Hmgu} page

Mutation origin

• Mutant Cell Line: EUCE320f12
• Germline Transmission: Earliest citation of germline transmission: J:275367
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: rsFlipROSAbgeo*
• Mutation details: A gene trap cassette flanked by inversely oriented pairs of the heterotypic FRT/F3 and lox511/loxP recombinase target sequences was inserted in intron 1 of the gene. Expression of FLP recombinase will invert the gene trap from its sense (mutagenic) orientation to antisense, allowing expression of the gene (floxed allele); subsequent Cre expression will reinduce the mutation by the reinversion of gene trap to its sense orientation, generating a knockout allele. (J:275367)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  EUCE320f12.p1ka3SPK	FI527163 ; (MGI Seq Detail)	3'Splinkerette PCR	Chr19:11783247-11783461 bp (-)
  EUCE320f12.q1k5SPK	FI536977 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr19:11783472-11783623 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stx3 Mutation: 18 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)