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Targeted Allele Detail
Symbol: Chat/Slc18a3tm1.1Vpra
Name: choline acetyltransferase; targeted mutation 1.1, Vania Prado
MGI ID: MGI:4367608
Synonyms: VAChTdel
Gene: Chat  Location: Chr14:32408203-32465909 bp, - strand  Genetic Position: Chr14, 19.4 cM
Germline Transmission:  Earliest citation of germline transmission: J:153908
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intergenic deletion
Mutation detailsOne loxP sequence was added 260 bp upstream from the translational initiation codon of Slc18a3 (within the first intron of Chat), and a second loxP was added approximately 1.5 kb downstream from the stop codon. This flanking region included the second exon of Chat. The TK-Neo cassette was added immediately after the second loxP and was followed by a third loxP (all in the same direction). The floxed region (including the TK-Neo cassette) was excised from mice by in vivo cre mediated recombination. No Slc18a3 mRNA was detected in homozygous mouse brain by qPCR. Slc18a3 protein was undetected in homozygous mutant mice by Western blot analysis of spinal cord extracts and immunostaining of the neuromuscular junction. Chat protein level was increased in E18.5 spinal cords and Chat mRNA level was increased as detected by qPCR from E18.5 mouse brains. (J:153908)
Generation of the Chat/Slc18a3tm1Vpra, Chat/Slc18a3tm1.1Vpra and Chat/Slc18a3tm1.2Vpra alleles
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 22 assay results
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Chat Mutation:  13 strains or lines available
Original:  J:153908 de Castro BM, et al., The vesicular acetylcholine transporter is required for neuromuscular development and function. Mol Cell Biol. 2009 Oct;29(19):5238-50
All:  4 reference(s)

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MGI 6.12
The Jackson Laboratory