Ptprc^tm1(cre)Medv
Targeted Allele Detail

Summary

• Symbol: Ptprc^tm1(cre)Medv
• Name: protein tyrosine phosphatase receptor type C; targeted mutation 1, Alexander Medvinsky
• MGI ID: MGI:4367466
• Synonyms: CD45-Cre
• Gene: Ptprc Location: Chr1:137990599-138103446 bp, - strand Genetic Position: Chr1, 60.73 cM
• Alliance: Ptprc^tm1(cre)Medv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152523
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Ptprc^tm1(cre)Medv expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ptprc (MGI:97810)

• Mutation details: The Cre recombinase encoding cDNA sequence, followed by the SV40 polyadenylation signal and a FRT-flanked puromycin resistance cassette, was inserted at the ATG translation initiation site in the second exon of the protein tyrosine phosphatase, receptor type, C (CD45) gene. The selection cassette was deleted by transient expression of FLPe in the correctly targeted ES cells. In mice bearing this allele and a GFP reporter transgene, fluorescence was observed only in CD45⁺ cells in the bone marrow, thymus, spleen and peripheral blood; the proportion of fluorescing cells of a given type appeared to correlate with the strength of CD45 expression. (J:152523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ptprc (mouse) Summary of all recombinase alleles driven by Ptprc. Alliance mouse Ptprc gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptprc Mutation: 188 strains or lines available

References

• Original: J:152523 Yang J, et al., Transgenic tools for analysis of the haematopoietic system: knock-in CD45 reporter and deletor mice. J Immunol Methods. 2008 Sep 15;337(2):81-7
• All: 5 reference(s)