Themis^tm1.1Hko
Targeted Allele Detail

Summary

• Symbol: Themis^tm1.1Hko
• Name: thymocyte selection associated; targeted mutation 1.1, Haruhiko Koseki
• MGI ID: MGI:4359650
• Synonyms: Themis^gfp
• Gene: Themis Location: Chr10:28544356-28759814 bp, + strand Genetic Position: Chr10, 16.06 cM
• Alliance: Themis^tm1.1Hko page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152601
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct containing GFP and a floxed neomycin cassette were used to replace exon 1 of the gene locus. The neomycin cassette was removed after targeting. GFP expression was detected in the double-positive and single-positive thymocytes as determined by flow cytometry. Endogenous protein was not expressed in homozygote thymocytes. (J:152601)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Themis Mutation: 55 strains or lines available

References

• Original: J:152601 Kakugawa K, et al., A novel gene essential for the development of single positive thymocytes. Mol Cell Biol. 2009 Sep;29(18):5128-35
• All: 1 reference(s)