Pdx1^tm1.1Stof
Targeted Allele Detail

Summary

• Symbol: Pdx1^tm1.1Stof
• Name: pancreatic and duodenal homeobox 1; targeted mutation 1.1, Doris Stoffers
• MGI ID: MGI:4359453
• Synonyms: Pdx1^deltaC
• Gene: Pdx1 Location: Chr5:147206907-147212658 bp, + strand Genetic Position: Chr5, 86.84 cM
• Alliance: Pdx1^tm1.1Stof page

Pancreatic hypoplasia in Pdx1^tm1Cvw/Pdx1^tm1.1Stof mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:152582
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Not Applicable) Mutations: Insertion, Nucleotide substitutions Mutation details: Exon 2 was replaced with one in which nucleotide substitutions result in the amino acid substitution of serine with a STOP codon at position 210 (S210X). The modified exon was followed by a floxed neo cassette that was removed via germ line, cre mediated recombination. The predicted product lacks 74 amino acids of the C terminus. A 75% reduction in full-length expression was confirmed by western blot analysis on E13.5 pancreatic extracts. (J:152582)

Generation of the Pdx1^tm1.1Stof allele

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdx1 Mutation: 35 strains or lines available

References

• Original: J:152582 Oliver-Krasinski JM, et al., The diabetes gene Pdx1 regulates the transcriptional network of pancreatic endocrine progenitor cells in mice. J Clin Invest. 2009 Jul;119(7):1888-98
• All: 2 reference(s)