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Ifnb1tm2.1Lien
Targeted Allele Detail
Nomenclature
Symbol: Ifnb1tm2.1Lien
Name: interferon beta 1, fibroblast; targeted mutation 2.1, Stefan Lienenklaus
MGI ID: MGI:4355683
Synonyms: IFN-Bflox beta-luc, IFN-Bfloxbeta-luc
Gene: Ifnb1  Location: Chr4:88522025-88522774 bp, - strand  Genetic Position: Chr4, 41.91 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:151854
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA loxP site was inserted 42 bp upstream of the lone coding exon and a Frt-flanked neomycin cassette, another loxP site, and a myc-tagged luciferase coding sequence were inserted downstream of the exon in the BstAPI restriction site. After targeting in C57BL/6 ES cells, the neomycin cassette was removed by crossing founder mice with FLP-deleter mice. A poly-A sequence upstream of the luciferase cassette prevents transcription of the reporter until the endogenous exon is floxed. (J:151854)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ifnb1 Mutation:  3 strains or lines available
References
Original:  J:151854 Lienenklaus S, et al., Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo. J Immunol. 2009 Sep 1;183(5):3229-36
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/30/2015
MGI 5.22
The Jackson Laboratory