Themis^tm1Gasc
Targeted Allele Detail

Summary

• Symbol: Themis^tm1Gasc
• Name: thymocyte selection associated; targeted mutation 1, Nicholas R J Gascoigne
• MGI ID: MGI:4353091
• Synonyms: Themis^-
• Gene: Themis Location: Chr10:28544356-28759814 bp, + strand Genetic Position: Chr10, 16.06 cM
• Alliance: Themis^tm1Gasc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151074
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette replaced exon 1 of the gene locus. Gene inactivation was confirmed by a lack of protein detected in immunoblot assays of homozygote thymus extract. (J:151074)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Themis Mutation: 55 strains or lines available

References

• Original: J:151074 Fu G, et al., Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. Nat Immunol. 2009 Aug;10(8):848-56
• All: 5 reference(s)