Mnd1^{Gt(RRS590)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Mnd1^{Gt(RRS590)Byg}
• Name: meiotic nuclear divisions 1; gene trap RRS590, BayGenomics
• MGI ID: MGI:4332240
• Synonyms: Mnd1^-, Mnd1^RRS590
• Gene: Mnd1 Location: Chr3:83995240-84063084 bp, - strand Genetic Position: Chr3, 37.46 cM, cytoband F1
• Alliance: Mnd1^{Gt(RRS590)Byg} page

Mutation origin

• Mutant Cell Line: RRS590
• Germline Transmission: Earliest citation of germline transmission: J:207221
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A beta-galactosidase-neomycin cassette was inserted 926 bp into intron 5. Western blot and RT-PCR analyses of testis cell extracts showed an absence of MND1 protein expression. (J:207221)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRS590	CG887207 ; (MGI Seq Detail)	5' RACE	Chr3:84023734-84063035 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  8 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mnd1 Mutation: 14 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)