Lmf1^{Gt(OST195742)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Lmf1^{Gt(OST195742)Lex}
• Name: lipase maturation factor 1; gene trap OST195742, Lexicon Genetics
• MGI ID: MGI:4226427
• Synonyms: LMF1^-
• Gene: Lmf1 Location: Chr17:25798059-25881800 bp, + strand Genetic Position: Chr17, 12.7 cM, cytoband A3.3
• Alliance: Lmf1^{Gt(OST195742)Lex} page

Mutation origin

• Mutant Cell Line: OST195742
• Germline Transmission: Earliest citation of germline transmission: J:223457
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene-trap cassette containing a splice acceptor sequence, neomycin resistance gene and polyadenylation sequence (SA-neo-pA), was inserted into intron 1. Although RT-PCR analysis revealed transcripts corresponding to exon 1 and the gene-trap cassette in neonatal liver, sequences 3' of exon 1 were undetectable in homozygous mutant mice. qPCR analysis showed absence of exon 6 expression in mutant MEFs, thus confirming absence of transcripts with sequences downstream of the integration site. The gene-trap allele most likely represents a null allele.
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST195742	CG568619 ; (MGI Seq Detail)	3' RACE	Chr17:25804528-25804828 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lmf1 Mutation: 30 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)