Lyar^{Gt(RRG292)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Lyar^{Gt(RRG292)Byg}
• Name: Ly1 antibody reactive clone; gene trap RRG292, BayGenomics
• MGI ID: MGI:4128951
• Synonyms: Lyar^gt
• Gene: Lyar Location: Chr5:38377815-38391650 bp, + strand Genetic Position: Chr5, 20.29 cM
• Alliance: Lyar^{Gt(RRG292)Byg} page

Mutation origin

• Mutant Cell Line: RRG292
• Germline Transmission: Earliest citation of germline transmission: J:263530
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The molecular lesion is a gene trao insertional mutation in intron 1 of the gene, upstream of the start codon in exon 2. End-point PCR analysis confirmed generation of the predicted Lyar-betageo fusion transcript in the spleen of homozygous mice. However, trace amounts of normally spliced transcript (exon 1exon 2) were also detected. Western blot analyses showed that low levels of encoded protein are detectable in spleen, thymus, pancreas, and teste of homozygous mice suggesting that this is a hypomorphic allele. (J:263530)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRG292	CC325828 ; (MGI Seq Detail)	5' RACE	Chr5:38377927-38377975 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  12 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lyar Mutation: 30 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)