Mpdz^Gt(XG734)Byg
Gene trapped Allele Detail

Summary

• Symbol: Mpdz^Gt(XG734)Byg
• Name: multiple PDZ domain crumbs cell polarity complex component; gene trap XG734, BayGenomics
• MGI ID: MGI:4125903
• Synonyms: Mpdz^{Gt(XG734)Byg(+/-)1KB}
• Gene: Mpdz Location: Chr4:81196742-81361052 bp, - strand Genetic Position: Chr4, 38.0 cM
• Alliance: Mpdz^Gt(XG734)Byg page

Mutation origin

• Mutant Cell Line: XG734
• Germline Transmission: Earliest citation of germline transmission: J:226456
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The insertional mutation by a gene-trapping vector, pGT1lxf, was designed to create an in-frame fusion between the 5' exons of the trapped gene and a reporter, geo (a fusion of beta-galactosidase and neomycin phosphotransferase II), and occurred in intron 11-12. The mutant yielded a fusion transcript containing exons 111 truncating the encoded protein after its third PDZ domain. Western blots of brain extracts from knockout homozygote pups confirmed the deletion and absence of full length encoded protein. (J:226456)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XG734	CC183060 ; (MGI Seq Detail)	5' RACE	Chr4:81287802-81296292 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mpdz Mutation: 79 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 8 reference(s)