Mcm2^{Gt(AB0178)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Mcm2^{Gt(AB0178)Wtsi}
• Name: minichromosome maintenance complex component 2; gene trap AB0178, Wellcome Trust Sanger Institute
• MGI ID: MGI:3863806
• Synonyms: Mcm2 (ABO178), Mcm2^Gt
• Gene: Mcm2 Location: Chr6:88860456-88875762 bp, - strand Genetic Position: Chr6, 39.64 cM
• Alliance: Mcm2^{Gt(AB0178)Wtsi} page

Mutation origin

• Mutant Cell Line: AB0178
• Germline Transmission: Earliest citation of germline transmission: J:165667
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The gene trap vector inserted into exon 10. Quantitative RT-PCR confirmed a 50% decrease in transcript expression in heterozygous mouse embryonic fibroblasts. (J:165667)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AB0178	CZ258650 ; (MGI Seq Detail)	5' RACE	Chr6:88864826-88865248 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  24 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcm2 Mutation: 49 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)